No you can have as many as you want. Though I’m not entirely sure how they work if loaded dynamically. That was a very easy and small thing which happens quite often when starting with the nav mesh… I didn’t do a lot of work there so I’m not sure of a lot of breakpoints and what to care about. Sorry :S
Found the issue . There is a begin play event in the AI blueprint that was not being called in the sub level . I added an extra event in level blueprint and it is working .